
农业与技术 ›› 2026, Vol. 46 ›› Issue (2): 166-171.DOI: 10.19754/j.nyyjs.20260228030
• 园林园艺 • 上一篇
龙杨 陈己任
出版日期:2026-02-28
发布日期:2026-02-28
作者简介:龙杨(2000-),女,硕士在读。研究方向:观赏园艺月季分子植物育种;通信作者陈已任(1972-),男,博士,教授。研究
方向:观赏植物种质资源与品种改良,观赏植物分子生物学等。
基金资助:Online:2026-02-28
Published:2026-02-28
摘要: RUBY是一种基于甜莱红素(betalain)生物合成的植物可视化报告系统,通过在同一转录本中表达关键 酶,使不含甜菜红素的植物在表达组织中可合成并积累红色甜菜红素,从而实现无需荧光设备或化学底物的肉眼 检测。自2020年首次系统报道以来,RUBY在模式植物与多种农作物中被成功应用于转化筛选、单倍体鉴别与 表型标记,其低成本、非侵入性的特点使其在育种与现场检测中具备显著优势。然而,UBY的模块长度、宿主 代谢负担与色素在不同组织/物种间的累积差异仍是其推广应用的主要挑战。因此,模块最小化、定量化判读与 多色/多模态可视化策略被认为是未来重要发展方向。
中图分类号:
. RUBY植物可视化报告系统的研究进展与应用前景[J]. 农业与技术, 2026, 46(2): 166-171.
| [1]Jefferson R A.Assaying chimeric genes in plants:the GUS gene fusion system J].Plant Molecular Biology Reporter,1987,5 (4):387-405. [2]Chalfie M,Tu Y,Euskirchen G,et al.Green fluorescent protein as a marker for gene expression [J].Science,1994,263 (5148): 802-805. [3]Ow D W,Wood K V,DeLuca M,et al.Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants[J].Science,1986,234(4778):856-859. [4]Cermak T,Curtin S J,Gil-Humanes J,et al.A multipurpose toolkit to enable advanced genome engineering in plants [J.The Plant Cell,2017,29(6):1196-1217. [5]He Y,Zhang T,Sun H,et al.A reporter for noninvasively monitoring gene expression and plant transformation [J.Horticulture Research,2020,7. [6]Wang D,Zhong Y,Feng B,et al.The RUBY reporter enables efficient haploid identification in maize and tomato [J].PlantBiotechnology Joural,2023,21 (8):1707-1715. [T]黄晨,张威,任红旭.植物中甜菜色素的研究进展[J].西北 植物学报,2023,43(12):2149-2160. [8]Polturak G,Breitel D,Grossman N,et al.Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants [J].New Phytologist, 2016,210(1):269-283. [9]Gandia-Herrero F,Garcia-Carmona F.Biosynthesis of betalains: yellow and violet plant pigments [J].Trends in Plant Science, 2013,18(6):334-343. [10]Pramanik D,Lee K,Wang K.A simple and efficient method for betalain quantification in RUBY-expressing plant samples [J]. Frontiers in Plant Science,2024,15:1449409. [11]Donnelly M LL,Hughes L E,Luke G,et al.The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like'sequences [J].Joumal of General Virology,2001,82(5):1027-1041. [12]Khan M I,Giridhar P.Plant betalains:Chemistry and biochemistry [J].Phytochemistry,2015,117:267-295. [13]Chen L,Cai Y,Liu X,et al.The RUBY reporter for visual selection in soybean genome editing J].Abiotech,2024,5 (2):209-213. [14]Tabara M,Matsumoto A,Kibayashi Y,et al.Straightforward and affordable agroinfiltration with RUBY accelerates RNA silencing research [J].Plant Molecular Biology,2024,114 (3):61. [15]Cermak T,Baltes NJ,Cegan R,et al.High-frequency,precise modification of the tomato genome [J].Genome Biology,2015, 16(1):232. [16]Yuan G,Lu H,Weston D J,et al.Reporter genes confer new- to-nature oramental traits in plants [J].Horticulture Research, 2022,9:uhac077.[17]Ge X,Wang P,Wang Y,et al.Development of an eco-friendly pink cotton germplasm by engineering betalain biosynthesis pathway [J].Plant Biotechnology Journal,2022,21 (4):674. [18]Deng Y J,Duan A Q,Liu H,et al.Generating colorful carrot germplasm through metabolic engineering of betalains pigments [J].Horticulture Research,2023,10 (4):uhad024. [19 Yu J,Deng S,Huang H,et al.Exploring the potential applications of the noninvasive reporter gene RUBY in plant genetic transformation [J].Forests,2023,14 (3):637. [20]张艾宁.DR5:RUBY和E8:RUBY报告系统在番茄果实颜 色改良中的应用[D].哈尔滨:东北农业大学,2024. [21 Lyu K,Zhang X,Yu W,et al.Optimization of hairy root transformation and application of RUBY as a reporter in Lotus comiculatus [J].Agronomy,2024,14 (6):1335. [22]Yao X,Taheri A,Liu H,et al.Improvement and application of vacuum-infiltration system in tomato [J].Horticulture Research, 2024,11(9):uhae197. [23]Lu J,Lu S,Su C,et al.Tissue culture-free transformation of traditional Chinese medicinal plants with root suckering capability [J].Horticulture Research,2024,11 (2):uhad290.[24]Kumar R,Kamuda T,Budhathoki R,et al.Agrobacterium-and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass J].Frontiers in Genome Editing,2022,4:960414. [25]Szymezak A L,Vignali D AA.Development of 2A peptide-based strategies in the design of multicistronic vectors[J.Expert Opinion on Biological Therapy,2005,5 (5):627-638. [26]Voinnet O,Rivas S,Mestre P,et al.Retracted:An enhanced transient expression system in plants based on suppression of gene silencing by the pl9 protein of tomato bushy stunt virus [J].The P1 ant Joumal,2003,33(5):949-956. [27]Miguel M G.Betalains in some species of the Amaranthaceae family:A review [J].Antioxidants,2018,7 (4):53. [28]Sadowska-Bartosz I,Bartosz G.Biological properties and applications of betalains [J].Molecules,2021,26 (9):2520. [29]Gand ia-Herrero F,Escribano J,Garc ia-Carmona F.Biological activities of plant pigments betalains [J].Critical Reviews in Food Science and Nutrition,2016,56 (6):937-945. [30]Nielsen J,Keasling J D.Engineering cellular metabolism [J]. Cell,2016,164(6):1185-1197. [31]Murphy K M,Ludwig E,Gutierrez J,et al.Deep leaming in image-based plant phenoty"[J ]Annual Review of Plant Biol0,2024,75(1):771-795. [32]Sheehan H,Feng T,Walker-Hale N,et al.Evolution of 1- DOPA 4,5-dioxygenase activity allows for recurrent specialisationto betalain pigmentation in Caryophyllales [J.New Phytologist, 2020,227(3):914-929. [33 Polturak G,Aharoni A.Advances and future directions in betalain metabolic engineering J].New Phytologist,2019,224 (4): 1472-1478. [34]Jung S,Maeda H A.Debottlenecking the L-DOPA 4,5- dioxygenase step with enhanced tyrosine supply boosts betalain production in Nicotiana benthamiana [J].Plant Physiology, 2024,195(3):2456-2471. [35]Sharifova S,Prasad K V S K,Cheema A,et al.Genetically encoded betalain based RUBY visual reporters:noninvasive monitoring of biological processes [J].Trends in Plant Science, 2025. [36]Baltes N J,Gil-Humanes J,Cermak T,et al.DNA replicons for plant genome engineering [J].The Plant Cell,2014,26 (1): 151-163. [37]Watson A,Ghosh S,Williams M J,et al.Speed breeding is a powerful tool to accelerate crop research and breeding [J.Nature P1anls,2018,4(1):23-29. [38]Thomsen P T,Meramo S,Ninivaggi L,et al.Beet red food colourant can be produced more sustainably with engineered Yarrowia lipolytica [J].Nature Microbiology,2023,8 (12): 2290-2303. |
| [1] | . 基于村民场所依恋的农业文化遗产地景观感知研究及提升策略[J]. 农业与技术, 2026, 46(2): 150-155. |
| [2] | . 多要素协同更新理念下历史文化街区的保护策略研究[J]. 农业与技术, 2026, 46(1): 176-185. |
| [3] | . 盐碱地雪菊种植技术要点与发展建议[J]. 农业与技术, 2026, 46(1): 192-195. |
| [4] | . 牡丹 PoSAUR 基因家族生物信息学分析[J]. 农业与技术, 2025, 45(23): 114-121. |
| [5] | . 基于自然暴露和公园可达性差异分析的徐州绿地布局优化研究[J]. 农业与技术, 2025, 45(23): 122-129. |
| [6] | . 长株潭绿心昭山示范区景观视觉美学质量评价研究[J]. 农业与技术, 2025, 45(22): 112-118. |
| [7] | . 双碳背景下肇庆市砚阳湖公园植物配置策略研究[J]. 农业与技术, 2025, 45(22): 124-127. |
| [8] | . 植物群落三维绿量对公园绿地生态效益的影响[J]. 农业与技术, 2025, 45(22): 128-134. |
| [9] | . 金丝皇菊分生苗规范化生态栽培种植技术[J]. 农业与技术, 2025, 45(21): 64-67. |
| [10] | . 野生花卉研究现状分析[J]. 农业与技术, 2025, 45(21): 113-117. |
| [11] | . 园艺文化养老在我国城市空间的应用模式及发展路径[J]. 农业与技术, 2025, 45(21): 118-123. |
| [12] | . 基于KANO-AHP模型的老I旧小区公共设施安全性评价[J]. 农业与技术, 2025, 45(21): 124-128. |
| [13] | . 基于网络点评数据的大运河杭钢公园后工业文化感知分析[J]. 农业与技术, 2025, 45(20): 106-112. |
| [14] | . 基于MSPA和MCR模型的潇贺古道江永段文化生态遗产廊道构建与分析[J]. 农业与技术, 2025, 45(20): 113-119. |
| [15] | . 地域文化视角下的城乡交错带景观提升设计[J]. 农业与技术, 2025, 45(20): 126-129. |
| 阅读次数 | ||||||
|
全文 |
|
|||||
|
摘要 |
|
|||||