蒙古沙冬青热激蛋白H$P基因家族鉴定及热迫表达分析

doi:10.19754/j.nyyjs.20250330018

摘要/Abstract

摘要： 植物因频繁暴露于多变的环境条件之下，常遭受非生物胁迫（如极端溫度一高温与低温、干旱、土壤盐碱化以及重金属离子污染)和生物胁迫（包括病原体侵染和害虫侵袭等）的双重挑战。这些极端环境因素严重影响植物的正常生长发育过程。蒙古沙冬青(Ammopi冲ptanthus mongolicus)隶属豆科沙冬青属常绿阔叶灌木，被列为国家重点保护植物和稀有树种。热激蛋白(heat shock protein,HSP)是植物生长发育过程中的一种必需蛋白，在植物响应逆境中起着至关重要的作用。为鉴定蒙古蒙古沙冬青HSP热激蛋白在热胁迫过程中的响应，本研究基于蒙古沙冬青全基因组数据，利用生物信息学分析的方法，共鉴定出了156个蒙古沙冬青HSP基因家族成员，且这些基因都不均匀的分配在9个染色体上。按照其基因结构，保守基序特征将其分为6个亚族，包括 HSP20、HSP60、HSP70、HSP90、HSP100-1和HSP100-2,根据系统进化树各亚族成员均在同一个分支且蛋白结构高度保守。通过对蒙古沙冬青45℃热胁迫不同时间(0h、3h、6h、12h、24h、48h)的转录组数据进行分析，筛选出SP基因家族中的差异基因成员共有98个，通过热图分析其在不同处理时间的差异表达情况。本研究可为蒙古沙冬青在耐热遗传育种方面的改良提供基因信息和理论基础。

中图分类号:

. 蒙古沙冬青热激蛋白H$P基因家族鉴定及热迫表达分析[J]. 农业与技术, 2025, 45(6): 87-92.

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