恩施富硒地区鱼腥草的遗传多样性与群体结构分析

doi:10.19754/j.nyyjs.20241230009

摘要/Abstract

摘要： 鱼腥草(Houttuynia cordata Thunb.)是一种食同源的中药材，具有丰富的营养和药用价值。中国湖北省恩施土家族苗族自治州因其丰富的硒矿藏而被誉为“世界硒都”。然而，恩施富硒地区鱼腥草的遗传特性尚不清楚，不利于该地区鱼腥草的开发和利用。本研究使用SS分子标记分析了恩施地区4个鱼腥草居群共96株个体。在群体结构分析中，SHHC02和HTHC04之间的遗传分化最大，SHHC01和SHHC03之间的遗传分化最小。遗传变异主要存在于居群间。同时，通过研究可以推断，该地区的4个居群可能起源于5个祖先亚群。因此，可以推断恩施富硒区的鱼腥草具有较高的遗传多样性，群体结构较为复杂，遗传组成较为多样，为该地区鱼腥草资源的后续利用和保护提供了依据。

中图分类号:

. 恩施富硒地区鱼腥草的遗传多样性与群体结构分析[J]. 农业与技术, 2024, 44(24): 37-42.

参考文献

1]Qing L,Ping Hong M,Da Wei J,et al.Comprehensive Assess- ment of Houttuynia cordata Thunb.,an Important Medicinal Plant and Vegetable ]Agronomy,2022,12 (10):2582. [2]Gupta S,Bharalee R.Genetic diversity and population structure of a medicinal herb Houttuynia cordata Thunb.of North-East India [J].Plant Molecular Biology Reporter,2021,39 (2):434- 442. [3]Gupta S,Bharalee R.Genetic Diversity and Population Structure of a Medicinal Herb Houttuynia cordata Thunb.of North-East India [J].Plant Molecular Biology Reporter,2020 prepublish):1-9. [4]Yang L,Jiang J G.Bioactive components and functional properties of Hottuynia cordata and its applications [J].Pharmaceutical Biol- 0,2009,47(12):1154-1161. [5]Xu Y W,Lei L,Dan Z,et al.Aliphatic aldehyde rich volatile constituents of Houttuyania cordata from southwest ChinaJ.Jour- nal of Medicinal Plant Research,2011,5 (24):5844-5847. [6]Jiangang F,Ling D,Zhang L,et al.Houttuynia cordata Thunb: a review of phytochemistry and pharmacology and quality control [J.Chinese Medicine,2013. [7]陈龙，彭程琪，樊明旭，等。鱼腥草药理作用及抗肺炎作用研究进展[J].人参研究，2022,34(05)：52-54. 8 Chang N,Luo Z,Li D,et al.Indigenous uses and pharmacologi- cal activity of traditional medicinal plants in Mount Taibai,China J.Evidence -Based Complementary and Alternative Medicine, 2017,2017(1):8329817. [9]洪纬。食物、药物和景物：鱼腥草在传统中国的利用[J刀].中国农史，2016,35(06)：111-120. [10]Tian TZ,Yu HJ,Zhen A C,et al.Novel Integrated Tiered Cu- mulative Risk Assessment of Heavy Metals in Food Homologous Tra- ditional Chinese Medicine Based on a Real-Life-Exposure Scenario [J.Frontiers in Pharmacology,2022. [11]张剑.食品中硒的功效与安全性分析[J].食品安全导刊， 2018(22):56-57. [12]Shuai Q,Lingyan Z,Yongzheng P,et al.Quality and Metabolo- mics Analysis of Houttuynia cordata Based on HS-SPME/GC-MS. [J.Molecules Basel,Switzerland),2022,27 (12):3921. [13]Felsenstein J.PHYLIP:Phylogeny interference package ver 3. 5c)[J.Department of Genetics,Univ,1993. [l4]根井，正利.Molecular population genetics and evolution[M]. North-Holland Pub.Co.,1975. [15]Peakall R O D,Smouse P E.GENALEX 6:genetic analysis in Excel.Population genetic software for teaching and research [J]. Molecular ecology notes,2006,6 (1):288-295. [16]Felsenstein J.PHYLIP Phylogeny Inference Package)version 3.6.[J.Department of Genome Sciences,University of Wash- ington,Seattle,2005. [17]Sudhir K,Glen S,Michael L,et al.MEGA X:Molecular Evo- lutionary Genetics Analysis across computing platforms [J].Mo- lecular Biology Evolution,2018 (6):6. [18]Letunic I,Bork P.Interactive Tree Of Life (iTOL)4:recent updates and new developments[J].Nucleic acids research, 2019,47(W1):256-259. [19]Pritchard J K,Stephens M J,Donnelly P J.Inference of Popula- tion Structure Using Multilocus Genotype Data J].Genetics, 2000,155(2):945-959. [20]Evanno G,Regnaut S,Goudet J.Detecting the number of clus- ters of individuals using the software STRUCTURE:a simulation study [J].Molecular ecology,2005,14 (8):2611-2620. [21]Earl D A,VonHoldt B M.STRUCTURE HARVESTER:a web- site and program for visualizingSTRUCTURE output and implemen- ting the Evanno method[J.Conservation genetics resources, 2012,4：359-361. [22]Jakobsson M,Rosenberg N A.CLUMPP:a cluster matching and permutation program for dealing with label switching and multimo- dality in analysis of population structure [J].Bioinformatics, 2007,23(14):1801-1806. [23]Rosenberg N A.distruct:a program for the graphical display of population structure [J ]Molecular Ecology Notes,2004,4 (1). [24]Loveless M D,Hamrick J L.Ecological determinants of genetic structure in plant populations [J].Annual review of ecology and systematics,1984:65-95. [25]李爱民，徐刚红，沈美珍。鱼腥草的花粉活力及雌雄配子体的发育[J].植物科学学报，2014,32(06)：561-566. [26]钟军，王坤，仇萍，等.鱼腥草不同地理群体遗传结构与变异的研究[J.草业学报，2011,20(02)：227-233. [27]Ueno S,Rodrigues J F,Alves-Pereira A,et al.Genetic varia- bility within and among populations of an invasive,exotic orchid [J].AoB Plants,2015,7:77. [28]Panda S,Naik D,Kamble A.Population structure and genetic diversity of the perennial medicinal shrub Plumbago [J].AoB Plants,2015,7:48. [29]Ying-Xiong Q,Min Z,Yu Y,et al.Genetic variation in wild and cultivated rhizoma corydalis revealed by ISSRs markers.J]. Planta medica,2009,75（1):94-98. [30]De Vita A,Bemardo L,Gargano D,et al.Investigating genetic diversity and habitat dynamics in Plantago brutia Plantaginace- ae),implications for the management of narrow endemics in Medi- terranean mountain pastures [J].Plant Biology,2009,11 (6): 821-828.[31]Jun H,Hong W,De-Zhu L,et al.Genetic diversity of Paris polyphylla var.yunnanensis,a traditional Chinese medicinal herb, detected by ISSR markers [J].Planta medica,2007,73 (12): 1316-1321. [32]Kun S,Wen C,Ruijun M,et al.Genetic variation in Hippophae rhamnoides ssp.sinensis Elaeagnaceae revealed by RAPD markers.[J].Biochemical genetics,2006,44 (5-6):186- 197. [33]Wei L,Wu X.Genetic Variation and Population Differentiation in a Medical Herb Houttuynia cordata in China Revealed by Inter- Simple Sequence Repeats (ISSRs)[J].IJMS,2012,13 (7): 8159-8170. [34]刘文龙，张喜利，贺福元，等.同一GAP产地鱼腥草株间基因多态性研究[J].湖南中医药大学学报，2011,31(11)： 30-33. [35]彭帅，伍贤进，罗玥佶，等.17份鱼腥草种质亲缘关系的S SR分析[J].安微农业科学，2007(12)：3484-3486. [36]聂志蛟.鱼腥草基因多态性与其挥发油指纹图谱变异关系的研究[D].长沙：湖南中医药大学，2009. [37]吴卫，郑有良，陈黎，等.鱼腥草种质资源的RAPD分析 [J].药学学报，2002(12)：986-992. [8]黎晓英，魏麟，伍贤进，等.中国不同地理居群鱼腥草遗传多样性分析[J].中草药，2010,41(02)：285-288. [39]蓝云龙，吴令上，袭波音，等.鱼腥草RAPD分子标记的多态性[J].浙江林学院学报，2008(03)：309-313. [40]Caetano-anolles G.High genome-wide mutation rates in vegeta- tively propagated bermudagrass [J].Molecular Ecology,1999,8 (7):1211-1221. [41]Wolf A T,Howe R W,Hamrick J L.Genetic diversity and popu- lation structure of the serpentine endemic Calystegia collina Conv- olvulaceae)in norther California [J].American Joumal of Bota- ny,2000,87(8):1138-1146. [42]Gross C L,Nelson P A,Haddadchi A,et al.Somatic mutations contribute to genotypic diversity in sterile and fertile populations of the threatened shrub,Grevillea rhizomatosa Proteaceae )J]. Annals of Botany,2012,109(2):331-342. [43]Barazani O,Westberg E,Hanin N,et al.A comparative analy- sis of genetic variation in rootstocks and scions of old olive trees-a window into the history of olive cultivation practices and past genetic variation [J].BMC Plant Biology,2014,14:1-8. [44]Gaut B S,Diez C M,Morrell P L.Genomics and the contrasting dynamics of annual and perennial domestication [J].Trends in genetics,2015,31(12):709-719. [45]Pelsy F,Dumas V,Bevilacqua L,et al.Chromosome replace- ment and deletion lead to clonal polymorphism of berry color in gra- pevine.[J].PLoS Genetics,2015,11 (4):1005081. [46]Foster M T,Aranzana J M.Attention sports fans!The far-reac- hing contributions of bud sport mutants to horticulture and plant bi- ology [J].Horticulture Research,2018,5 (1):1-13. [47]H C S B.Influences of clonality on plant sexual reproduction. [J].Proceedings of the National Academy of Sciences of the Unit- ed States of America,2015,112(29):8859-8866. [48]Moore G A.Oranges and lemons:clues to the taxonomy of Citrus from molecular markers [J].TRENDS in Genetics,2001,17 (9):536-540. [49]This P,Lacombe T,Thomas R M.Historical origins and genetic diversity of wine grapes J].Trends in Genetics,2006,22 (9):511-519. [50]Frederique P,Stephanie H,Ximena M,et al.An extensive study of the genetic diversity within seven French wine grape variety collections.[J].TAG.Theoretical and applied genetics.Theore- tische und angewandte Genetik,2010,120 (6):1219-1231. [51]关金枝.湖北省鱼腥草种质资源的遗传多样性研究[D].武汉：华中农业大学，2010. [52]Gupta M,Gupta S.An Overview of Selenium Uptake,Metabo- lism,and Toxicity in Plants [J].Frontiers in Plant Science, 2017. [53]孙元鹏，袁知详，刘于思，等。乡村振兴战略下恩施州道地药材高质量发展的探讨[J].浙江农业科学，2019,60 (08)：1408-1412. [54]尹文仲，杨云贵.恩施自治州鱼服腥草的资源现状及发展简介 [C].中国自然资源学会天然药物资源专业委员会，陕西省科学技术厅，陕西省食品药品监督管理局，陕西省陕南中药产业发展领导小组，中国药材GAP研究促进会（香港）.全国第六届天然药物资源学术研讨会论文集.湖北民族学院附属医院：来凤县地产药材有限责任公司，2004：2. [55]Wu Z,Bafuelos G S,Lin Z Q,et al.Biofortification and phy- toremediation of selenium in ChinaJ.Frontiers in plant sci- ence,2015,6:136. [56 Zhao B,Zhou S,Wu X,et al.Distribution and Accumulation of Selenium in Plants and Health Risk Assessment from a Selenium- Rich Area in China [J.Polish Joural of Environmental Studies, 2018,27(6). [57]Lv L,Li L,Zhang R,et al.Effects of dietary supplementation of selenium enriched yeast on egg selenium content and egg production of north China hens [J].Pak J Zool,2019,51 (1):49-55. [58]Zhang L,Song H,Guo Y,et al.Benefit-risk assessment of di- etary selenium and its associated metals intake in China (2017- 2019):Is current selenium-rich agro-food safe enough?[J]. Joumal of Hazardous Materials,2020,398:123224.

[1]	. 贝母属植物叶绿体基因组研究进展[J]. 农业与技术, 2024, 44(24): 10-14.
[2]	. 小麦转录因子TaWRKY27-6A生物信息学分析及功能初探[J]. 农业与技术, 2024, 44(24): 15-18.
[3]	. 基于16 SrRNA扩增子序列研究平凉地区冬小麦根际土壤微生物群落多样性[J]. 农业与技术, 2024, 44(23): 20-26.
[4]	. 中国农业碳足迹压力时空分异及其预测分析[J]. 农业与技术, 2024, 44(23): 61-68.
[5]	. 建设用地健康风险评估有关探讨[J]. 农业与技术, 2024, 44(23): 69-72.
[6]	. 经济发达省域碳排放责任核算与优化[J]. 农业与技术, 2024, 44(23): 79-84.
[7]	. 生态产品价值实现内涵模式及其前瞻[J]. 农业与技术, 2024, 44(23): 85-88.
[8]	. 长株潭绿心视觉美学质量及视觉异质性研究[J]. 农业与技术, 2024, 44(23): 93-98.
[9]	. 我国乡村振兴示范县空间分布特征及其驱动因素研究[J]. 农业与技术, 2024, 44(22): 88-95.
[10]	. 梯度扩散薄膜技术在土壤环境中的应用进展[J]. 农业与技术, 2024, 44(22): 104-107.
[11]	. 汾河流域A级旅游景区空间分布及影响因素[J]. 农业与技术, 2024, 44(22): 108-112.
[12]	. 基于三调数据的沿海城市生态系统服务价值估算[J]. 农业与技术, 2024, 44(22): 113-116.
[13]	. 东北边境县域的城镇化格局及其驱动力[J]. 农业与技术, 2024, 44(21): 62-67.
[14]	. 安徽省生态系统服务与城镇化时空相关性分析[J]. 农业与技术, 2024, 44(21): 68-73.
[15]	. 平凉市水土保持区划思路与成果分析评价[J]. 农业与技术, 2024, 44(21): 74-78.